Technology

Millions of fragments to be sequenced massively and in parallel

Our platform uses Illumina's NextSeq2000 system, which is based on sequencing by synthesis (SBS), where tagged nucleotides are tracked simultaneously with DNA strand copying.

This technology allows millions of fragments to be sequenced massively and in parallel, improving the speed and accuracy of sequencing while reducing its cost.

The Illumina NextSeq 2000Sequencing System has been provided with a novel high-resolution optical system that produces high-precision image data with higher resolution and sensitivity than more traditional Illumina systems. This technology also provides greater sequencing flexibility, scalable to different production experimental needs, and adaptable to both conventional and emerging applications.


Applications

The technology and the services that GENVIP offers through this Platform provides particularly important advantages for mid-throughput sequencing options. Some of the most common applications of this system are the complete sequencing of the human exome, the sequencing of mRNA and the sequencing of panels of genes or specific targets.

The flexibility of the technology makes the platform adaptable to a wide range of applications and applicable to projects with diverse sequencing objectives.

Transcriptomic Sequencing

The Nextseq 2000 System offers great flexibility for RNA sequencing studies, which require different sequencing depths and read lengths depending on study complexity, size, and objectives:

Total RNA sequencing

Analyze the coding regions and multiple forms of non-coding RNA to get a complete picture of the transcriptome.

mRNA sequencing

Detect and quantify new and known transcripts for complete and accurate expression profiles.

Gene expression profiles

It allows multiple RNA libraries to be sequenced together in a single sequencing run, obtaining a rapid picture of the most expressed genes with only a few reads per sample.

Targeted RNA sequencing

Select and sequence transcripts of specific interest for gene expression profiling studies.

Sequencing of small RNA (RNAs) and MicroRNA (miRNA)

It allows queries for thousands of RNA and microRNA sequences with high sensitivity.

Sequencing of the human exome and microorganisms genome

Sequencing of small genomes

Sequencing of he entire genome of bacteria, virus, or other patogens.

Sequencing of the human exome

Capture and sequencing the protein coding region of the genome, a cost-effective alternative to “whole genoma” sequencing.

Targeted panel sequencing

The gene targeting and gene targeting panels sequence genomic or transcriptomic regions of interest. They are useful tools to analyze specific mutations, mutational load, microsatellite instability, HLA genotyping, expression of the TCR repertoire or gene fusion profiles. Applications of targeted panel sequencing include:

Personalized genomic sequencing

Selection and sequencing of specific regions of the genome that have a specific research interest: for example, specific biological pathways or follow-up experiments from genome-wide association studies or whole genome sequencing.

Pre-designed panels for targeted gene sequencing

They contain fixed genomic regions or genes associated with a disease, phenotype, or biological pathway. They are available for the investigation of various diseases such as cancer, hereditary disorders, rare disorders, cardiac disorders, neurological disorders, immune diseases.

Other potential applications

Free Circulating DNA Sequencing and Liquid Biopsy Analysis

Chromatin analysis (ChIP-Seq, ATAC-Seq)

DNA methylation sequencing

16S ribosomal RNA (rRNA) and internal transcribed spacer (ITS) sequencing

Metagenomic profiles (metagenomics, metatranscriptomics)

Single cell DNA and RNA sequencing

The Service

Our service includes all the stages of support that your project needs, from the advisory service and experimental advice, to the technical support for the development of sequencing protocols, material management, sample analysis and the corresponding analysis of bioinformatic data for its publication, if aplicable.

Single Cell service

What is Single Cell?

This technology from 10x Genomics allows toreveal and study full complexity of cellular diversity, from different -omicperspectives at a single cell level: transcriptomic, proteomic, epigenomic andcombining information from different -omic layers to elevate the power ofyour  experiments.

Chromium X Single Cell platform to furtherexpand services from our NGS service

Advantages of 10x Genomics technology:

  • High-throughput single-cell sequencing
  • Target thousands of cells per sample. This results in a low cost per cell in the case of high-throughput projects.
  • Multi-dimensional single-cell data. Multiple products that can be combined to obtain single-cell data with multiple dimensions.

Several options:

  • Single Cell Gene Expression: It allows you to measure the 3’ gene expression for 3,000 –10,000 cells per sample and identify different cell types, determine cellular heterogeneity of your sample and compare sample before and after treatment.
  • Single Cell Immune Profiling: for analyzing both the T-cell and B-cell receptor, cell surface protein expression, antigen specificity, and/or CRISPR edits, along with gene expression, all from a single cell.
  • Single Cell ATAC-seq: allows users to profile the regulatory landscape of chromatin in thousands of cells.
  • Single Cell Multiome ATAC and Gene Expression: multiomic profiling of the transcriptome and chromatin accessibility from the same cell enhances the characterization of cell types and states, and gain deeper insights into underlying gene regulatory mechanisms.Targeted Gene Expression. It allows to analyze the expression of a defined set of transcripts relevant to an specific research area or interest at a single cell resolution

The NextGeneration of Single Cell now available!

The Chromium X Series lets access single cell analysis at any stage. It offers cost-effective, lower throughput single cell analysis, this make single cell analysis a perfect fit to perform any 10xGenomics single cell assay at the scale you need for your project, with the highest throughput flexibility—for analysis of hundreds to hundreds of thousands of cells per run.

Contactus and we will help you to meet your research goals!

Contact

Experimental Advisory Service

Our sequencing platform provides experimental advice for a wide variety of research projects requiring:

  • Total RNA sequencing
  • mRNA sequencing
  • Sequencing of small RNAs and miRNAs
  • Gene expression profiles
  • Complete sequencing of small genomes
  • Human whole exome sequencing (based on enrichment technology)
  • Sequencing of specific gene panels (based on PCR amplicon or enrichment technology)
  • Sequencing of ctDNA, Chip-Seq, ATAQ-seq and any other genomic application, with prior evaluation of the application.

Technical support

We offer technical support for the full development of the sequencing protocol:

  • Experimental design of sequencing projects
  • Extraction of nucleic acids from different types of samples: blood, saliva, bone or tissue embedded in formalin fixed paraffin (FFPE).
  • Quality control and sample integrity
  • Preparation and quality control of the NGS libraries
  • Sequencing of NGS libraries

Materials management

We provide the management and ordering of the required reagents for the preparation of the NGS libraries, flow cells and cartridges for the specific sequencing application.

Bioinformatics data analysis

We deliver the results in sequencing file formats containing the raw data and offer specialized processing and analysis of NGS data: quality control, de-multiplexing, alignment and post-analysis. We are experts in designing the experiment matching exactly your needs

About us

The GENVIP research group is a multidisciplinary team of a transversal nature on different disciplines in the field of clinical and basic research. GENVIP uses cutting-edge technologies and analytical methods from different disciplines to optimize the treatment of pediatric patients and the innovative approach to infectious diseases from the point of view of the host. The main objective of the GENVIP research group is to gain understanding of the pathogenesis of infectious diseases that affect children from different perspectives, through an "-omics" approach.

This strategy helps us identify genetic, proteomic and transcriptomic biomarkers that allow us to define signatures associated with the diagnosis or prognosis of different pediatric infectious pathologies based on their molecular phenotype.

In addition, this approach allows us to obtain and analyze information that is used translationally in clinical practice, contributing to early diagnosis, selective prevention and treatment of pediatric patients.

Contact

For information about the GENVIP service “Genome sequencing, transcriptomics and data analysis platform”, please contact:

Antonio Salas Ellacuriaga
antonio.salas@usc.es

Alberto Gómez Carballa
alberto.gomez.carballa@sergas.es

Isabel Ferreirós Vidal
isabel.ferreiros.vidal@sergas.es